Coding

Part:BBa_K1595029

Designed by: Jonlin Chen   Group: iGEM15_Cornell   (2015-09-13)

EDA and GSG linker

T7 promoter + ribosome binding site + EDA + GSG + BamHI + NdeI + 6xHist tag + Terminator. Coding device for KDPG aldolase (EDA), a peptide-stabilizing protein, and a Gly-Ser-Gly linker sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 729
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 641
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None